Chloramphenicol acetyltransferase cat
WebThis 500-bp enhancer directs expression of the bacterial chloramphenicol acetyltransferase (CAT) gene in a differentiation-dependent fashion when linked to its own minimal promoter or to an enhancerless SV40 promoter. Moreover, this enhancer stimulates very strong and highly specific expression from the CAT gene in the adipose tissues of ... WebChloramphenicol-Acetyltransferase (oder CAT) ist ein bakterielles Enzym (EC 2.3.1.28). [1] [2] Es acetyliert das Antibiotikum Chloramphenicol unter Verbrauch von Acetyl-CoA an zwei Stellen. Da acetyliertes Chloramphenicol nicht mehr an Ribosomen bindet und damit nicht mehr die Translation hemmen kann, vermittelt das Gen eine Resistenz gegen ...
Chloramphenicol acetyltransferase cat
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WebMar 31, 2015 · The chloramphenicol acetyltransferase resistance cassette, cat, located on the pACDK4-C plasmid backbone and originally used for selection in E. coli, was removed. Chloramphenicol is used as a secondary treatment option for RMSF, and genes conferring resistance are not suitable as recombinant material for rickettsial transformation. WebTo investigate the role of splicing in the regulation of gene expression, we have generated transgenic mice carrying the human histone H4 promoter linked to the bacterial gene for chloramphenicol acetyltransferase (CAT), with or without a heterologous intron in the transcription unit. We found that …
WebJan 13, 2012 · Widely used reporter genes typically include those encoding chloramphenicol acetyltransferase (CAT), β-galactosidase (lacZ), and neomycin phosphotransferase (neo); however, each of these suffers from one or more disadvantages including high backgrounds, costly and tedious assay procedures, and low signal-to … WebChloramphenicol acetyltransferase (or CAT) is a bacterial enzyme (EC 2.3.1.28) that detoxifies the antibiotic chloramphenicol and is responsible for chloramphenicol resistance in bacteria. This enzyme …
WebThe primary mechanism of resistance to chloramphenicol is due to the presence of chloramphenicol acetyltransferase (CAT), an enzyme which catalyzes the acetylCoA-dependent acetylation of the antibiotic at the C-3 hydroxyl group (58). Genes for CAT are widely distributed among most gram-negative and gram-positive bacteria and several …
WebHere, we used this approach, termed as thermoadaptation-directed enzyme evolution, to increase the thermostability of the chloramphenicol acetyltransferase (CAT) of Staphylococcus aureus and successfully generated a CAT variant with an A138T replacement (CAT(A138T)).
WebA transcriptional fusion between a promoterless chloramphenicol acetyltransferase (CAT) reporter gene cartridge and C. coli VC167 DNA carrying flaB transcription and translation … indian herbs specialities pvt ltd saharanpurWebNov 1, 2004 · 1 Chemical structures and properties of chloramphenicol and florfenicol. Chloramphenicol (Cm), originally referred to as chloromycetin, was isolated from … local weather 19971WebMar 1, 2024 · Riemerella anatipestifer causes serositis and septicaemia in domestic ducks, geese, and turkeys. Traditionally, the antibiotics were used to treat this disease. Currently, our understanding of R. anatipestifer susceptibility to chloramphenicol and the underlying resistance mechanism is limited. In this study, the cat gene was identified in 69/192 … indian herbs near meWebOf the many different reporter genes available for gene transfer studies in mammalian cells, the chloramphenicol acetyltransferase (CAT) gene ( 5) has probably been the most widely used. Another reporter gene that is frequently employed is the E. coli β-galactosidase gene ( 6 ). The E. coli xanthine-guanine phosphoribosyltransferase ( 7, 8 ... local weather 19966WebAn Escherichia coli CM2555 strain, sensitive to chloramphenicol when expressing the cat gene and producing active chloramphenicol acetyltransferase (CAT), was described recently. It was proposed that this sensitivity is due to decreased levels of acetyl coenzyme A (Acetyl CoA) in cat-expressing CM2555 cells in the presence of chloramphenicol. local weather 1966WebA very popular reporter gene and protein has been chloramphenicol acetyltransferase or CAT. This is a bacterial gene that evolved to neutralize the antibiotic … indian herb that helps joint painWebFolding of chloramphenicol acetyltransferase (CAT) in Escherichia coli is hampered by deletion of the carboxy-terminal tail including the last residue of the carboxy-terminal alpha-helix. Such truncated CAT polypeptides quantitatively aggregate into cytoplasmic inclusion bodies, which results in absence of a chloramphenicol-resistant phenotype ... local weather 20623