Gallic acid calibration curve
WebQuantitative determination of total phenols was done on the basis of a standard curve of gallic acid and linearity of the calibration curve was achieved between 6.25 to 100 … WebFor Trolox and ascorbic acid, method sensitivity, given by the slope of the calibration curves (min μM −1), was similar for both the AUC and lag time approaches . However, …
Gallic acid calibration curve
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WebApr 9, 2024 · Phenolic compounds are frequently present in various natural products, and they can have different beneficial biological potentials. The most widely used method for determination of individual phenolic compounds is high-performance liquid chromatography (HPLC). In this paper, a method for simultaneous determination of 16 phenolic … WebMolecules 2013, 18 6855 The percentage increase in absorbance of each solution was compared at the wavelengths of 691, 715, 760, and 800 nm. The statistical analysis indicated a significant increase in absorbance at 760 nm compared to data for 691 and 715 nm for all solutions (gallic acid, F60,3P < 0.001; tannic acid, F60,3P < 0.001; catechin, …
WebJul 18, 2024 · The presence of ursolic acid and rosmarinic acid was found in both solvents. However, gallic acid, quercetin, ... was measured at 750 nm using a microplate reader (Bio-Rad, iMark, Hercules, CA). Distilled water was used as a blank. Calibration curves were performed with 5 to 150 mg gallic acid/L as a standard, and methanol (80% v/v) was … WebStandard Gallic Acid Test Lab Report. RESULTS AND DISCUSSION: Graph 1 Calibration curve for Standard Gallic acid A standard curve of gallic acid was constructed to …
WebOct 23, 2024 · HPLC determination of β-Sitosterol and Gallic acid in seeds of Nigella sativa was carried out using reversed-phase Grace C18 column (250 mm × 4.6 mm id, 5 µm … WebSep 10, 2024 · TPC in samples was estimated by gallic acid calibration curve, and results were expressed as mg of gallic acid equivalent (GAE)/100 g dry mass of plant material (Kamtekar et al., 2014). Total Flavonoid Content Assay. TFC of each extract was determined by using aluminum chloride colorimetric assay (Kamtekar et al., 2014).
WebMay 6, 2024 · From this stock solution of GA, you can now prepare a calibration curve by adding 0, 0.1, 0.2, 0.3, 0.5, 1.0 ml of the GA stock …
WebThe absorbance was measured at 760 nm. Gallic acid was used as standard for the calibration curve and was plotted at 0.02, 0.04, 0.06, 0.08, and 0.10 mg·mL −1, prepared in methanol-water (50:50, v/v). TPC values were determined using an equation obtained from the calibration curve of gallic acid graph (R 2 = 0.9990). brays island plantation south carolinaWebthe different concentration of gallic acid only used to to establish the calibration curve this curve is only used for getting the regression equation in the form of y=ax+b … brays lake rolla moWebMar 27, 2013 · The calibration curve was constructed by putting the value of absorbance vs. concentration. A similar procedure was adopted for the extracts as above described … corsicana tax officeWebTrolox® and/or gallic acid. Calibration curves were determined (relative standard deviation was within the range from 1.5 to 2.5 %). The obtained characteristics were compared and discussed. Moreover, the data obtained were applied to optimize and to automate all mentioned protocols. Automatic analyzer allowed us to analyse … corsicana texas businessesWebApr 20, 2024 · The maximum absorption wavelength of gallic acid was determined to be 765 nm from these spectra and a calibration curve of gallic acid was prepared at 765 nm. The total phenolic content of endemic Asparagus lycicus plant was calculated by using gallic acid calibration curve (Spanos and Wrolstad 1990). brays island realty llcWebApr 17, 2024 · Gallic acid can be used as such a standard antioxidant, and the resulting EACs are calculated as calibration curve slopes divided by the slope for gallic acid. In [ 14 ] the immobilization of iron(III)–Phen complex on Nafion membrane led to two-fold decrease of EAC for ascorbic acid. brays landing washingtonWebNov 22, 2024 · The total polyphenol content was determined as the equivalent of gallic acid (mg g −1 extract) based on the gallic acid calibration curve (y = 0.0217x + 0.0561, R 2 = 0.9673). 3.10. Total Flavonoid Content. The flavonoid assay was performed by using a modified version of the method reported by Pękal et al. . In a 96-well plate, 70% EtOH ... corsicana texas airport