Hiscript® ii one step rt-pcr kit
WebbThe Vazyme HiScript II One Step qRT-PCR SYBR Green Kits are designed for SYBR Green I-based qPCRs that directly use RNA as templates. The reverse transcription and PCR can be finished in one tube, significantly reducing pipetting procedures and the risk of … Webb22 mars 2024 · The QIAGEN OneStep RT-PCR Kit provides a convenient format for highly sensitive and specific RT-PCR using any RNA template. The kit includes optimized components that allow both reverse transcription and PCR amplification to take place in the same reaction mix in a "one-step" reaction.
Hiscript® ii one step rt-pcr kit
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WebbThis one-step RT-PCR kit allows efficient, specific cDNA synthesis and amplification in a single tube for endpoint analysis (e.g., gel electrophoresis). The kit features a streamlined workflow that does not require addition of reagents between the cDNA synthesis and amplification steps. Webb2 × One Step U+ Mix One Step U+ Enzyme Mix 50 × ROX Reference Dye 1 Gene Specific Primer Forward (10 µM) Gene Specific Primer Reverse (10 µM) TaqMan Probe (10 µM) 模板RNA 实验流程(以ABI StepOnePlus为测试机型) to 20 µl 10 µl 1 µl 0.4 µl 0.4 µl 0.4 µl 0.2 µl Total RNA: 1 pg - 1 μg 2.按下列条件进行One Step qRT-PCR ...
WebbHiScript II Reverse Transcriptase, RNase inhibitor and Champagne Taq plus DNA Polymerase. Storage Store at -30 ~ -15℃ and transport at ≤0℃. Applications It is … WebbHiScript III One Step qRT-PCR Probe Kit is specially designed for qPCR that directly use RNA (e.g. virus RNA) as template. Using gene specific primers (GSP), the reverse …
WebbRT-PCR qPCR · · Ace-Hi One Step qPCR-PCR SYBR Green Kit Ace-Hi One Step qPCR-PCR Probe Kit · · ClonExpress II One Step Cloning Kit ClonExpress MultiS One Step Cloning Kit 克 隆 到 克 目 隆 的 到 载 体 载 体 T · · · · · 重组后的质粒DNA · WebbHiScript II One Step RT-PCR Kit (Dye Plus)专为以RNA为模板(如RNA病毒)的终点法PCR检测而设计，PCR产物可直接加样电泳，大大节 省了操作人工耗时。 使用基因特 …
Webb2O 2 × One Step SYBR Green Mixa One Step SYBR Green Enzyme Mixb 50 × ROX Reference Dye 1c 50 × ROX Reference Dye 2c 2 × 1.25 ml 2 × 1.25 ml 250 μl 100 μl 100 μl Components Web: www.vazymebiotech.com Tel: +86 25-83772625 Email: [email protected] Add: Red Maple Hi-tech Industry Park, Nanjing, PRC Version 22.1
Webb15 juni 2024 · HiScript® II One Step RT-PCR Kit (Dye Plus) 一步法高效RT-PCR试剂盒 逆转录和PCR在同一管内进行 检测灵敏度可达0.1 pg总RNA 可扩增长达10 kb以上的长片段 PCR产物可直接加样电泳 本试剂盒专为以RNA 为模板 ( 如RNA 病毒) 的终点法PCR 检测而设计。 使用基因特异引物 (GSP)，逆转录和PCR 反应在一管内完成，不需要额外的开 … guittard unsweetened chocolate baking chipsWebb10 apr. 2024 · Virus copy numbers were determined by RT-qPCR, using a HiScript II One Step qRT-PCR SYBR Green Kit (Vazyme Biotech, Nanjing, China). Primers were designed based on the N and sgE gene sequences of SARS-CoV-2, and the viral RNA load in the lung tissues was determined by the TaqMan fluorescent quantitative PCR … guitteny 44WebbHiScript III U+ One Step qRT-PCR Probe Kit. HiScript III U+ One Step qRT-PCR Probe Kit专为以RNA为模板（RNA病毒）的定量PCR检测而设计。. 整合HiScript III Reverse Transcriptase、RNase inhibitor以及热启动的Champagne Taq DNA Polymerase的优越性能，配合经过优化的缓冲体系，逆转录和qPCR反应在一管内 ... guittard white satinWebbHiScript II One Step RT-PCR Kit . 一步法高效RT-PCR 试剂盒. 病毒检测；基因表达分析；终点法PCR检测. · 逆转录和PCR 在同一管内进行. · 检测灵敏度可达1 pg 总RNA. · … guittard white chipsWebb22 mars 2024 · QIAGEN OneStep RT-PCR Buffer has been specially developed to allow both efficient reverse transcription and PCR amplification. The buffer contains novel … Vi skulle vilja visa dig en beskrivning här men webbplatsen du tittar på tillåter inte … guittard white pastel ribbonWebbSuperScript II Reverse Transcriptase (RT) is an improved version of SuperScript RT. ... GC-Rich PCR Performance. High. PCR Method. 1-step RT-qPCR, 2-step RT-qPCR. Reaction Speed. 50 min. Technique. Reverse Transcription. Optimal Reaction Temperature. 42°C. Quantity. 2,000 Units. guittard white chocolate ribbonsWebbRT-PCR qPCR · · Ace-Hi One Step qPCR-PCR SYBR Green Kit Ace-Hi One Step qPCR-PCR Probe Kit · · ClonExpress II One Step Cloning Kit ClonExpress MultiS One Step … guitton hopital foch suresnes 64