WebFeb 24, 2024 · To mimic the oral mucosa and submucosa, we employed human primary gingival (keratinocyte and stomal) cells; specifically, stromal cells were embedded in type I collagen extracellular matrix (ECM) to provide structural integrity inside the scaffold (Figure 1), while keratinocytes were seeded on the closed porosity of the scaffold. WebJun 25, 2024 · The current study presents the effect of naked Fe3O4@Carbon nanoparticles obtained by the combustion method on primary human gingival fibroblasts (HGFs) and primary gingival keratinocytes (PGKs)—relevant cell lines of buccal oral mucosa. In this regard, the objectives of this study were as follows: (i) development via combustion …
Oral Free Full-Text Biological Impact of the Ratio of E-Cigarette ...
Webdifferent manner. Primary gingival keratinocytes, have an epithelial morphology, with some round form, cobblestone appearance and strong adhesion qualities which at highest concentrations utilized (5, 10, and 25 µg/mL) lose their shape and viability, as can be observed in figure 1. The same observations can be made in the case of fibroblasts: WebAug 15, 2010 · Upon commitment of gingival keratinocytes to terminal differentiation, a number of biochemical changes occur, namely, the expression of cytokeratins 5, 14 and 10 . Cytokeratins 5 and 14 are normally expressed in the basal cells of gingival stratified epithelium and have been used as proliferative cell markers [ 28 - 30 ]. cryptomichnl twitter
Engineering a 3D In Vitro Model of Human Gingival Tissue
Webgingival keratinocytes, gingival fibroblasts and periodontal ligament fibroblasts by using the direct explant technique; and (2) to compare the effect of sex and age of those who provided tissue samples for the successful tissue cultures. 2. Materials and Methods 2.1Collection of tissue samples Gingival tissue for primary gingival keratinocytes WebBecause the gas can make contact with primary gingival cells during the ozonation process, both human gingival fibroblasts and keratinocytes were exposed to different doses of … WebPrimary human gingival keratinocytes isolated in CnT-Prime media benefit from the presence of PCT factors that help establish as many proliferative progenitors as possible … cryptomich twitter