WebMar 21, 2024 · The structure of a DNA-strand transfer complex of mouse RAG1-RAG2 at 3.1-A resolution is determined. In analyzing site-specific DNA cleavage by the mouse RAG1-RAG2 recombinase, which initiates V(D)J recombination, the study finds that the active site is reconfigured for the two consecutive reactions and the DNA double helix adopts … Web002216 B6.129S7- Rag1 tm1Mom /J Mice homozygous for the Rag1 tm1Mom mutation produce no mature T cells or B cells. Their phenotype can be described as a "non-leaky" immune deficiency. Of note, …
Frontiers CD4+ T cell memory is impaired by species-specific ...
WebMar 7, 2024 · Mice constitutively lacking all conventional MHC II genes (H2 dlAb1-Ea), 26 Rag1-deficient (Rag1 −/−) mice, 27 and Rag2-deficient (Rag2 −/−) mice 28 have been previously described. All mouse strains were on the B6 genetic background and maintained at the Francis Crick Institute’s animal facilities. how far back can i claim expenses
Recombination-Activating Gene 1 Knockout Mouse - ScienceDirect
WebFeb 10, 2024 · Mice deficient in recombination-activating genes 1 and 2 ( RAG1 and RAG2) are considered a robust model to study the immune response in whole organisms. This is … WebNov 12, 2012 · The second aim was to target the mouse and rat recombination activating gene 1 (Rag1) to describe, for the first time, Rag1 knockout immunodeficient rats. We microinjected a plasmid encoding a meganuclease for Rag1 into the pronucleus of mouse and rat zygotes. Mutant animals were detected by PCR sequencing of the targeted … WebApr 14, 2024 · Figure 2 Persistence of env-reactive CD4 + CTLs in lymphocyte-deficient hosts. (A) Flow-cytometric detection of CD44 + env-reactive donor CD4 + T cells (top) and TCF-1 + and GzmB + CD4 + T cells (bottom) in spleens of Rag1-/-recipient mice. (B) Absolute numbers (left), GzmB expression (middle) and TCF-1 expression (right) of env-reactive … hiding washing machine